Example Scripts for AutoScript TEM

This page contain example scripts for AutoScript TEM.  If you want your script to be added to this page, please contact us via autoscripttemsupport@thermofisher.com

Fresnel Free Imaging Mode (FFIM) for Transmission Electron Microscopy

Special thanks for the script to Nestor Zaluzec
Pritzker School of Molecular Engineering, University of Chicago, Chicago, Illinois, USA
Photon Sciences Directorate, Argonne National Laboratory, Lemont, Illinois, USA

For more please see “Fresnel Free Imaging Mode (FFIM) for Transmission Electron Microscopy: A Computationally Mediated Solution # using Spatio-Temporal Functionalization of the Illumination Optics”, Microscopy and Microanalysis, 30 (Suppl 1), 2024, 1634–1638. https://doi.org/10.1093/mam/ozae044.806

Supported HW: Spectra.

AutoScript 1.9 and higher.

Experimental variation of contrast in HREM imaging with FFIM mode Off (3a) and On (3b). Note the reduction of the superimposed Fresnel illumination contrast and the negligible effect on the local image contrast. From Microscopy and Microanalysis, 30 (Suppl 1), 2024, 1634–1638. https://doi.org/10.1093/mam/ozae044.806


Acquisition of EDS maps with rocking beam using combination of AutoScript and Velox

Special thanks for the script to Fumihiko Uesugi, National Institute for Materials Science, Tsukuba-shi Ibaraki, Japan.

For application also see Direct Mapping of Fluorine in Cation Disordered Rocksalt Cathodes by Krishna Prasad Koirala, Lin Jiang, Shripad Patil, Paolo Longo, Zhao Liu, Bert Freitag, Juri Barthel, Ethan C. Self, Yingge Du, Lee Pullan, Ted Tessner, Leslie J. Allen, Wu Xu, Wei Tong, Jagjit Nanda, and Chongmin Wang

Supported HW: Spectra, Talos.  Velox 3.12 or higher.

AutoScript 1.5 and higher.

Comparison of compositional mapping and rocking beam technique. In mapping the beam is moved across the
area of interest (left) while in rocking beam mode the stationary beam s tilted in both directions on the specimen (right).

Three rocking beam maps on STO in {100} projection with different tilt ranges (half angle of 40,60,80mrad)
are shown to illustrate the maximum tilt range (upper and middle graphs). The intensity ratio map of Sr/O and
Ti/O ICP (bottom images) reveal the elemental ratio changes with different beam to crystal orientation, which
can influence quantitative compositional analysis. (Channeling)


Example of the Atom detection in STEM with AutoScript TEM and AI

Supported HW: Spectra, Talos. Required PC setup is described in the downloadable package.

AutoScript 1.5 and higher.


Example of Particle detection in STEM with AutoScript TEM and AI

Supported HW: Spectra, Talos. Required PC setup is described in the downloadable package.

AutoScript 1.5 and higher.


Download example how to acquire STEM image from12 segments of Panther STEM and HAADF simultaneously

Supported HW: Spectra, Talos

AutoScript 1.1 and higher


Download example of the tile acquisition in TEM.  Please also check STEM tiled acquisition script (below) for more details.

Supported HW: Spectra, Talos

AutoScript 1.1 and higher


Download example of the tile acquisition in STEM

Supported HW: Spectra, Talos

AutoScript 1.1 and higher


Download example of the work with apertures

Supported HW: Spectra, Talos

AutoScript 1.1 and higher



Donwload example how to display image metadata

Supported HW: Spectra, Talos

AutoScript 1.1 and higher


Download example of focus series acquisition

Supported HW: Spectra, Talos

AutoScript 1.1 and higher


Download example how to acquire SmartCam image

Supported HW: Spectra, Talos

AutoScript 1.1 and higher